Journal: PLoS ONE

Article Title: Di (2-ethylhexyl) Phthalate Exposure Impairs Growth of Antral Follicle in Mice

doi: 10.1371/journal.pone.0148350

Figure Lengend Snippet: (A) apoptotic processes and (B) negative regulation of cell proliferation related genes expressions, via microarray, in control and DEHP-treatment groups.

Article Snippet: We used 6 μg of high quality RNA labeled with Cy5, and hybridized to a mouse oligo microarray (Phalanx Mouse Whole Genome One Array TM ; Phalanx Biotech Group, Palo Alto, CA, USA).

Techniques: Microarray, Control

Journal: PLoS ONE

Article Title: Di (2-ethylhexyl) Phthalate Exposure Impairs Growth of Antral Follicle in Mice

doi: 10.1371/journal.pone.0148350

Figure Lengend Snippet: The green bars represent gene expression quantities of control and DEHP-treatment groups microarray data, and the red bars represent qPCR data in DEHP-treatment groups compared with the control group. Compared to the control group, relative fold changes were presented as mean ± SD. All experiments were repeated at least three times independently. (* P < 0.05; ** P < 0.01).

Article Snippet: We used 6 μg of high quality RNA labeled with Cy5, and hybridized to a mouse oligo microarray (Phalanx Mouse Whole Genome One Array TM ; Phalanx Biotech Group, Palo Alto, CA, USA).

Techniques: Gene Expression, Control, Microarray

Journal: Steroids

Article Title: Membrane-Initiated Nuclear Trafficking of the Glucocorticoid Receptor in Hypothalamic Neurons

doi: 10.1016/j.steroids.2017.12.005

Figure Lengend Snippet: Differential transcriptional effects of Dex and Dex-BSA. A. N42 cells transfected with GRE-TK-Luciferase for 24 hours were treated with Dex or Dex-BSA for 20 min. Hormone was removed and replaced with fresh media for 24 hours before lysis. **, p < 0.01; ns, p=0.064 compared to vehicle, two-tailed t-test; vehicles: DMSO and cyclodextrin in water (CD). B. Venn diagram for gene expression profiles in N42 cells following 24 hours of Dex and Dex-BSA treatment, from a Mouse OneArray® microarray (Phalanx Biotech Group) on 3 independent biological replicates. Genes reported show a ≥ 0.5-fold difference in expression (p < 0.05) compared to vehicle-treated cells.

Article Snippet: B. Venn diagram for gene expression profiles in N42 cells following 24 hours of Dex and Dex-BSA treatment, from a Mouse OneArray® microarray (Phalanx Biotech Group) on 3 independent biological replicates.

Techniques: Transfection, Luciferase, Lysis, Two Tailed Test, Gene Expression, Microarray, Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Key Molecular Mechanisms of Chaiqinchengqi Decoction in Alleviating the Pulmonary Albumin Leakage Caused by Endotoxemia in Severe Acute Pancreatitis Rats

doi: 10.1155/2016/3265368

Figure Lengend Snippet: Predicting target proteins for CQCQD to attenuate pulmonary albumin leakage based on the analyses of rats pulmonary tissue microarray and also the construction of PPI network. (a) The proteins, encoded by these prioritized hub nodes including Fos, Pik3r1, and Src, were predicted as the key target proteins (red nodes = upregulated DEGs, green nodes = downregulated DEGs, grey nodes = other interactive genes; the size of nodes = the betweenness of the node). (b) The expressions of these 3 prioritized hub nodes in each sample within each group (red represents upregulation and green represents downregulation).

Article Snippet: According to the Rosetta Biosoftware, out of 20715 gene probes on the Rat OneArray Plus Microarray, 3190 gens (1678 genes upregulated and 1512 genes downregulated) in S/N DEGs cluster and 4138 genes (2404 genes upregulated and 1734 genes downregulated) in C/S DEGs cluster were differentially expressed ( P < 0.05, |log 2(Ratio)|≧0.585).

Techniques: Microarray